Objective:To study the prevalence,genotypes and molecular characteristics of norovirus (NV) in acute foodborne disease of Jiangsu Province. Methods:Feces or anal swab specimens of 16 658 patients with foodborne disease from 2013 to 2014 were collected and detected by RT-PCR. Positive samples were of randomly selected and analyzed by nucleotide sequence determination and phylogenetic tree analysis to determine the genotype. Meanwhile,we collected the relevant epidemiological data. Results:In the 16 658 samples,1 208 positive specimens were identified with NV(7.25%). Positive rate was 7.11% in males and 7.45% in females,which had no statistical significance(χ2=0.63,P=0.43). The prevalence of the children aged 1 to 4 years was the highest(9.85%). Prevalence time was mainly concentrated in October-the following year in April. A total of 63 out of the 71 positive specimens were classified,with 6(9.5%) belonged to GⅠ,including 3 GⅠ.3,2 GⅠ.9,and 1 GⅠ.4. Other 57(90.5%) all belonged to GⅡ.4. type. Conclusion:Norovirus is one of the most important pathogens causing foodborne diseases with the characteristics of wide distribution,prevalence time in winter and spring, and high risk incidence in children,and the harm caused by it should be paid more attention to. The main genotypes which caused NV are the GⅡ group,and the dominant strain of the present stage is GⅡ.4 type.