Objective:To describe the distribution of polymorphic site of the new short tandem repeat (STR) loci in Jiangsu Chinese Han population. Methods:A total of 9 STR loci were amplified by STRtyper-10G Direct Kit and genotyped by Genetic Analyzer,and all samples were collected from 598 unrelated individuals in Jiangsu Han population during 2015. Results:A total of 111 genotypes were found,of which 13 were rare alleles. The frequency,greater than 0.08,was 13,of which the maximum frequency of “19/20” from D13S325 locus was 0.130 4. Heterozygosity was 0.851 ± 0.068,matching probability was 0.054 ± 0.022,polymorphism information content was 0.808 ± 0.052,power of discrimination was 0.946 ± 0.022 and probability of paternity exclusion was 0.697 ± 0.130. Conclusion:With the analysis of alleles in a large number of samples and frequencies,objective alleles of more genetic polymorphism data of Jiangsu population should be obtained,so that the need of further practical application could be met in the case of micro alterations.