Objective:To analyze polymorphism distribution of 12 non-CODIS STR(including D11S4463,D2S1776,D14S1434,D4S2408,D10S1043,D20S482,D12SATA63,D19S433,etc.)of Jiangsu population by 3130 genetic gene sequencing electrophoretograms,and to calculate the gene frequency(P),discrimination power(DP),heterozygosity(H),polymorphic information content(PIC),probability of exclusion of due-testing(PEduo)and probability of exclusion of trios-testing (PEtrio). And then,to evaluate its forensic application value. Methods:The products of PCR amplification mere detected by capillary electrophoresis usiny 3130 sequenator. Data were analyzed by analysis software. Results:The frequency distributions of the 12 non-STR CODIS loci were consistent with the Hardy-Weinberg equilibrium. H ranged from 0.707 6 to 0.804 6,DP was 0.846 8 to 0.948 6,PEduo was 0.278 6 to 0.482 6,PEtrio was 0.446 7 to 0.663 2,and PIC was 0.643 8 to 0.807 4. Conclusion:Through the statistics of a large amount of experimental samples and allele frequencies,the allelic polymorphism data were obtained for comparison and verification. It has a good auxiliary function and play an important complementary role in cases of CPI less than 10 000 and mutation.