Objective:To investigate the expressions and significances of mTORC1-HIF1α pathway genes in human CD8+ regulatory T cell. Methods:Coculture systerm of CD8+ T cells and SKOV3 were conducted in vitro,CD8+ T cells cultured alone group acted as control group. At day 5,CD8+ T cells were collected,and used to examine the expression of eight glycolysis genes by quantitative real-time reverse transcriptase(qRT)-PCR(mTORC1,HIF1α,Glut1,PKM2,GPI,TPI,Eno1,and LDHα). Western blot was used to detect the expression of mTORC1,HIF1α and PKM2. We collected peripheral blood from 14 ovarian cancer(OC) patients,12 benign diseases,and 12 healthy females. CD8+ T cells were then separated using a CD8-positive isolation kit. The expressions of mTORC1,HIF1α,Glut1,PKM2,GPI,TPI,Eno1,and LDHα in CD8+ T cells from OC patients was detected by qRT-PCR and compared with that in patients with ovary benign tumor(BOT)and healthy volunteers. Results:Compared with CD8+ T cells cultured without SKOV3 cells,glycolysis gene expression showed varying degrees of decline in CD8+ T cells cultured with SKOV3 cells. The expression of mTORC1,HIF1α,PKM2,GPI,and TPI was significantly decreased in co-cultured cells compared with the control group. Results showed that the expression of mTORC1,HIF1α,and PKM2 decreased significantly(P < 0.05)in CD8+ T cells cultured with SKOV3 cells compared with control group. HIF1α and Glut1 mRNA had lower expression levels in CD8+ T cells from OC patients than those from healthy controls(both P < 0.05). mTORC1,PKM2,GPI,and TPI were also expressed at lower levels in OC patients than those in either BOT patients or healthy controls(both P < 0.05). The expression of mTORC1-HIF1α pathway genes has no difference between benign diseases group and the healthy controls. Conclusion:mTORC1-HIF1α signalling integrates the control of CD8+ T cells metabolism and differentiation in ovarian cancer microenvironment,which play a significant role in the immunotherapy of OC