Objective:To investigate the migration and invasion of lung neoplasm potentiated by MKL1 and the change of CCNB2 activity involved in signal pathway. Methods:Lung cancer cells were infected with lentivirus carrying indicated shRNAs and effect of MKL1 inhibition on migration and invasion of lung cancer cells was measured. Small interfering RNA was transfected to suppress the expression of MKL1,RT-PCR was performed to examine CCNB2 mRNA level,and luciferase reporter assays was to assess the effect of MKL1 deletion on the promoter activities of CCNB2. Results:Cell colony formation was diminished by soft agar clonogenic assay and cell proliferation was reduced when MKL1 was silenced by siRNA. MKL1 depletion directly repressed CCNB2 transcription,and the inhibition was up to 70%. RT-PCR showed great depression of CCNB2 mRNA by the interference of MKL1. Difference was of significance(P < 0.05). Conclusion:Depletion of MKL1 by shRNA significantly hampered the migration and invasion of lung cancer cell. MKL1 depletion inhibited CCNB2 transcriptional activity and repressed mRNA expression. The study provided early-stage markers for lung cancer diagnosis and treatment.