CD4+CD25+FoxP3+ regulatory T cell （Treg） plays an indispensable role in the maintenance of immune balance. In current clinical trials， Treg infusion has been proved to be an effective way to treat graft-versus-host disease （GVHD）. Therefore， how to improve Treg function， proliferation and survival by appropriate means is very important for basic and clinical immunology researchers. MicroRNAs （miRNAs） are a class of non-coding RNAs that can negatively regulate mRNA and inhibit the expression of target proteins. Several miRNAs have been shown to influence Treg function directly or indirectly. Here， we provide an overview of the relationships between these discovered miRNA signaling pathways and Treg cells， which might give a new insight into future basic and clinical studies.