Objective:To generate human monoclonal antibodies(mAbs)against the rabies virus glycoprotein(RVG)by immunizing the mice carrying human immunoglobulin transloci, and further to identify their characters. Methods:The human IgM transgenic mice were immunized with the rabies virus glycoprotein. The human anti-RVG hybridoma cell lines were screened and produced by using the classical hybridoma technique. The specificity and isotype of mAbs were determined by the double antibody sandwich enzyme- linked immunosorbent assay(ELISA). Also,the binding properties with the inactivated rabies virus CVS-11 were analyzed. Results:Five hybridoma cell lines were established,they can stably produce human anti-rabies virus IgM monoclonal antibodies(5D1,6H11,9A3,15D6,and 19E6). These mAbs specifically recognized the r-RVG and three human anti-r-RVG mAbs specifically combined with the inactivated rabies virus CVS-11 strain. Conclusion:Hybridoma cell lines were successfully established,and can stably produce human anti-rabies virus IgM monoclonal antibodies. Human anti-r-RVG mAbs could specifically combine with the inactivated rabies virus CVS-11 strain. This may be a foundation for further development of vaccine to prevent and control rabies.