Objective:To explore the molecular mechanism in which IL-8 receptor promoted gastric cancer metastasis. Methods:Gastric cancer cells were cultured and transfected with IL-8 receptor antisense expression plasmid pcDNA3.1(+)/as-IL-8G and IL-8 receptor sense expression plasmid pcDNA3.1(+)/s-IL-8G. Protein expression was detected by Western blotting. Cell invasion was performed in Transwell and the invasion capacity was expressed by the relative invasive cell number. Results:Gastric cancer cells with lower expression of IL-8 receptor had significantly reduced invasion ability,and the expression of c-Jun,Ets-1 and MMP-9 decreased correspondingly,while higher expression of IL-8 receptor markedly enhanced the invasion capacity of gastric cancer cells,and the expression of c-Jun,Ets-1 and MMP-9 increased significantly. MMP-9 protein expression level decreased significantly after blocking the c-Jun or Ets-1 expression by phosphorylated modification antisense oligonucleotide technology,and the cell invasion ability decreased obviously at the same time. Conclusion:IL-8 receptor promoted gastric cancer cells metastasis through abnormal c-Jun and Ets-1-mediated MMP-9.