Objective：To evaluate the diagnostic value of serum mesothelin level on ovarian cancer. Methods：Relevant studies were identified by systematically searching PubMed，Embase，Web of Science，CBMdisc，WANFANG，CNKI and so on，articles related to the diagnostic value of serum mesothelin on ovarian cancer were selected. Data analysis was conducted by RevMan 5.3，Stata 12.0 and Meta-Disc1.4. Results：Nine studies with a total of 451 ovarian cancer cases and 617 controls were included. The results of meta-analysis showed that sensitivity and specificity were 0.80 (95%CI：0.76～0.83) and 0.81 95%CI：0.78～0.84)，respectively;positive likelihood ratio and negative likelihood ratio were 6.93 (95%CI：3.32～14.46) and 0.24 (95%CI：0.16～0.35)，respectively;diagnostic odds ratio was 45.69 (95%CI：17.49～119.35);the area under curve of the summary receiver operating characteristic(SROC) was 0.90，Q=0.83. Conclusion：Mesothelin shows a high specificity and a low sensitivity in the diagnosis of ovarian cancer，and it can be used as an effective way for diagnosis of ovarian cancer combined detection with other tumor markers.