Objective: To explore the promotive effect of FOXC1 on the invasion of glioblastoma U87 cells. Methods: The mRNA and protein expression levels of endogenous FOXC1 was examined in U87 and U251 cells as well as NHAs via qPCR and Western blot, respectively. The FOXC1-siRNA was transfected into U87 cells. The transfection efficacy of FOXC1-siRNA was examined using qPCR and Western blot. The wound healing assay and Transwell invasion assay were carried out to investigate the role of FOXC1 on glioma cell invasion. The changes of epithelial-mesenchymal transition(EMT)-related signaling pathway proteins and marker proteins in glioma cells were identified with Western blot. Results: Downregulating the expression of FOXC1 reduced the invasive ability of glioma cells, reduced the EMT-related signaling pathway proteins of glioma cells, increased the epithelial biomarker E-cadherin and decreased the mesenchymal biomarkers N-cadherin and Vimentin. Conclusion: The FOXC1 could effectively promote the invasion of glioma cells by inducing EMT.