Objective:Using isobaric tags for relative and absolute quantitation（iTRAQ），to find the region-typical membrane proteins of cochlear nuclues（CN）.Methods:The subjects are male D60 SD rats.The four regions CN,Inferior Colliculus(IC) ,Superior Oliver Com-plex(SOC) and the rest of the brain(Rest) ,were seperated. The region-typical proteins of CN were identified with iTRAQ. The functions of these proteins and the process of biological metabolism involved were discussed seriously through Uniprot.Results:Seventeen region-typical proteins of CN were identified.GO(Gene Ontology) pathway analysis indicates that they are mainly involved in biological regulation processes and development. Gal-1（Galectin-1），ANXA2（Annexin-A2）， and PRPH（Peripherin）were found to be related to the function of the nervous system through Uniprot.Gal-1 was involved in neural development and synapase plasticity，and has neuroprotective effects. ANXA2 had neurotrophic effects, and might be involved in the release of neurotransmitters. Highly expressed PRPH led to neurotoxi-city, and was involed in neurodegenerative disease.Conclusion: In CN, Gal-1, ANXA2 and PRPH proteins might be related to central audi-tory processing, and it provides theoretical basis for further researches on the pathogenesis of central auditory processing disorder.