Objective：To explore related miRNAs to Mycoplasma pneumoniae(Mp) caused respiratory infections and immune reaction. Methods：Blood of children with Mp pneumonia(MPP) was collected. By miRNA microarray technology，miRNAs expression differences in lymphocytes were analysed between MPP and control group. RT-PCR was used to verify the accuracy of microarray results. Specific miRNAs associated with MPP were explored. Target gene software was used to predict the possible target genes，and RT-PCT was used to verify the mRNA expression difference. Results：Compared with normal group，in MPP group 105 miRNAs increased(with 30 more than 5 fold change，10 more than 10) and 133 decreased (with 133 less than 0.5 fold) (P＜0.05). Significantly differentially expressed miRNAs：miR-20a-3p，miR-100-5p，miR-93-5p，and let-7b-5p increased detected by RT-PCR，and were consistent with the array results. As potential target genes，Bcl-2，IGF-1R，PTEN，and TGFβR1 mRNA expression decreased. Conclusion：miRNAs differential expressed in blood lymphocytes in children with MPP，and the difference may be involved in infection and the immune response caused by Mp.