Objective： To investigate the difference of expression and prognostic significance of cyclin-dependent kinase 4 （CDK4） and Geminin in gliomas. Methods： Immunohistochemical was used to detect the expressions of CDK4 and Geminin in 70 human gliomas. Their relationships with clinicopathologic factors and survival time of patients were also analyzed. Results： The positive rates of CDK4 and Geminin in gliomas were 70.0%（49/70） and 75.7%（53/70），respectively. The positive rates of CDK4 in gradeⅡ， grade Ⅲ， grade Ⅳ of gliomas were 52.8%（21/36）, 84.6%（22/26） and 100.0%（8/8）, and the positive rates of Geminin in grad Ⅱ, Ⅲ and Ⅳ were 58.3%（21/36），92.3%（24/26） and 100.0%（8/8），respectively. A statistical significance of their expressions was observed among different grades （P<0.05）. The expressions of them were positively correlated. The positive expressions of Geminin and CDK4 were related to the tumor size and the number of nuclear division（P<0.05），and were not related to age，sex and necrosis of tumor tissue（P>0.05）. Kaplan-Meier univariate statistical analysis showed that the survival time of patients with positive CDK4 and Geminin expression group was significantly shorter than that of the negative CDK4 and Geminin expression group（P<0.001）. Conclusion： The expressions of CDK4 and Geminin are related to the grading of gliomas and prognosis. Combined detection of CDK4 and Geminin is helpful to judge the malignant degree and prognosis of gliomas.