Objective：To investigate the effects of ETS1/2 on pancreas development of Xenopus laevis. Methods：The specific morpholino oligonueleitides(MOs) were designed to knockdown the ETS1/2 by microinjection. The expression of marker genes was tested during pancreas formation by whole mount in situ hybridization and quantitative RT-PCR. ETS1/2 downstream genes were screened by bioinformatics analysis. Results：Knockdown of ETS1 or ETS2 had no effect on embryo development of Xenopus laevis. Knockdown of both ETS1/2 promoted the expression of pancreas marker genes while inhibited the expression of intestine marker genes，and had no effects on the expression of liver and stomach marker genes. The binding sites of ETS1/2 were found in the promoter regions of pancreatic marker genes ngn3，igf1 and foxa1. Conclusion：Knockdown of ETS1/2 promoted the pancreas development of Xenopus laevis while inhibited the intestine development，and had no effects on the development of the liver and stomach.