Objective：To investigate the impact of maternal high-protein diet on metabolism related hormones of rats offspring during pregnancy and lactation. Methods：Female Wistar rats (F0 generation) were mated and randomly assigned to three groups：ProⅠ，ProⅡ，ProⅢ，and were fed ad libitum with isocaloric purified diets：DietⅠ(protein，14%；carbohydrate，69.3%，E/E)，DietⅡ(protein，24%；carbohydrate，59.3%，E/E) and DietⅢ(protein，34%；carbohydrate，49.3%，E/E) respectively. On the 3rd day after birth (P3)，only 3 male rats (F1 generation) of each dam were kept. After 21 days of suckling period，rats of F1 generation from different groups were weaned onto the same normal diets till P77，when the research was over. Blood samples of offspring at P21/P49/P77 were kept and levels of hormone related to energy metabolism were detected. Results：Serum levels of IGF-1 of F1 generation were lower in ProⅠ than in ProⅡ(P＜0.01) at P21；IGF-1 levels were higher in both ProⅡ and ProⅢ than in ProⅠ(P＜0.01) at P49；IGF-1 levels of ProⅢ were higher than ProⅠ and ProⅡ(P＜0.01) at P77. Serum levels of Leptin of F1 generation were the highest in ProⅡ than in ProⅠ and ProⅢ(P＜0.01) at P21；Leptin levels of ProⅠ were lower than both ProⅡ and ProⅢ at P49 and P77(P＜0.05). Plasma levels of NPY of ProⅢ were lower than ProⅠ and ProⅡ(P＜0.01) at P49，with no differences between groups at P77(P＞0.05). Plasma levels of Ghrelin were higher in ProⅢ than in ProⅠ and ProⅡ (P＜0.01) at P77，with no differences between groups at P49(P＞0.05). Conclusion：Peripheral blood levels of leptin，ghrelin and IGF-1 were raised as a result of protein-rich diet during pregnancy and lactation in rats，with little influence on NPY levels.