Objective：To investigate the expression of EZH2 in laryngocarcinoma tissues and cells，and its effect on proliferation，migration and invasion of laryngocarcinoma HEP-2 and SCC10A cells. Methods：Thirty laryngeal squamous cell carcinoma tissues and 10 normal para-carcinoma tissues were collected from Xuancheng Central Hospital，AnHui Province. The expressions of EZH2 were compared between laryngocarcinoma tissues and the para-carcinoma tissues，as well as laryngocarcinoma HEP-2 and SCC10A cells by qRT-PCR. EZH2 shRNA was transfected into HEP-2 cells by Lipofectamine2000 and the transfection efficiency was examined by qRT-PCR. Cell proliferation was evaluated by CCK8 assay. The cell migration and invasion abilities were detected by migration and invasion assay. The tumorigenesis assay in nude mice was performed to test the role of EZH2 in the tumor growth of HEP-2 cells in vivo. Results：The expressions of EZH2 in laryngocarcinoma tissues and cells were both significantly higher than those in para-carcinoma tissues(P＜0.01). EZH2 shRNA was successfully transfected into HEP-2 cells. The proliferation ability of HEP-2 cells was decreased significantly after transfection of EZH2 shRNA. The migration and invasion of HEP-2 cells were significantly inhibited. The EZH2 shRNA suppressed tumor growth of HEP-2 cells in vivo. Conclusion：EZH2 is overexpressed in laryngocarcinoma tissues and cells. The proliferation，migration and invasion of human laryngocarcinoma HEP-2 cells are inhibited after the transfection of EZH2 shRNA. EZH2 shRNA suppresses tumorigenesis of HEP-2 cells in vivo.