Objective: To establish porcine naive-like induced pluripotent stem (iPS) cell line followed by labelling with the red fluorescent protein via nucleofection. The labelled porcine naive-like iPS cells could be used for tracing their development and differentiation. Methods: Firstly, bama miniature pig embryonic fibroblasts (PEF) were nucleofected with TetO-FUW-OSKM (OSKM: mouse derived transcriptional factors OCT4, SOX2, KLF4 and c-MYC) and FUW-M2rtTA vectors. Then, these cells were incubated in the medium with both leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF), supplemented with doxycycline hyclate. The established iPS cell lines were identified for their pluripotency. Secondly, the iPS cells were nucleofected with pEF1alpha-DsRed-Express Vector which could express the red fluorescent protein, then the maintainence of pluripotent characters of these labeled cells were analyzed. Results: The established iPS showed domed morphology and could survive long-term single-cell passaging (30 passages). They were alkaline-phosphatase positive, maintained a normal karyotype, expressed various pluripotency markers and required LIF/STAT3 signaling pathway for self renewal. The iPS cells could form embryonic bodies(EB), which expressed markers of three germ layers after culture in LIF free medium. The iPS cells were transfected with red fluorescent protein vector successfullyand kept showing red fluorescence for 10 passages. The results of alkaline phosphatase staining and immunofluorescence staining suggested that the pluripotency of these cells had not been compromised after red fluorescent protein labelling. Conclusion: The red fluorescent protein labeled porcine naive-like iPS cell lines were established successfully.