Objective: To investigate the expression of PARP14 and its biological functions in pancreatic ductal adenocarcinoma (PDAC). Methods: The public gene chip data of PDAC obtained from the Gene Expression Omnibus (GEO) database and the Cancer Genome Atlas (TCGA) database were used to analyze the expression of PARP14 between pancreatic tumors and its adjacent tissues, The Kaplan-Meier method and Log rank test were used to assess survival analysis according to PARP14 expression in PDAC from TCGA. The biological role of PARP14 in regulating the proliferation of PDAC cells was evaluated by CCK-8 assay and colony formation experiment in BXPC-3 and CFPAC-1 cells transfected with PARP14 siRNAs. Results: The expression level of PARP14 in PDAC tissues were higher than that in the adjacent tissues or healthy controls according to the GEO databases (P<0.001). TCGA database further revealed that higher expression of PAPR14 was significantly associated with poorer survival of PDAC patients (P<0.05). Besides, PARP14 siRNAs were successfully transfected into PDAC cells BXPC-3 and CFPAC-1, and transfection with PARP14 siRNAs significantly suppressed proliferation of PDAC cells (P<0.05). Conclusion: The expression of PARP14 is up-regulated in PDAC tissues and high expression of PARP14 predicts poor survival, suggesting its role as a potential oncogene in PDAC.