Objective：To verify changes of NF-κB in hypercoagulable state of rats and find new targets to treat thrombotic diseases. Methods：Eighty rats were splited into model group and control group. The model group was injected with adrenaline hydrochloride at the dosage determined in pre-experiment，while the control group was injected with normal saline. After 12，18，24，48 and 96 h，eight rats of each group were anaesthetized to collect blood sample. Preparing plasma samples were prepared to test PT，APTT， and the whole blood viscosity， and serum were collected to test the level of NF-κB. Results：In the pre-experiment，death rate of the model group of 0.7 mg/kg was lower，and compared with the control group，PT and APTT were shorter(P＜0.05) and the whole blood viscosity was higher(P＜0.05)；The model group showed distinct differences on PT and APTT compared with the control group at time of 12 h and 18 h(P＜0.05)，the whole blood viscosity of the model group is higher than that of the control group within 24 h，and the level of NF-κB of the model group is higher than that of the control group within 48 h. Conclusion：The best dosage of adrenaline is 0.7 mg/kg. Level of NF-κB in rats with hypercoagulable state is higher than that of the control group，which decreases with time. The hypercoagulable state in rats may be related to the increasing expression of NF-κB pathway.