A study of neuroprotective mechanism of serum miRNA-181a in Parkinson’s disease
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    Abstract:

    Objective:To investigate the neuroprotective mechanism of serum miRNA-181a on dopaminergic neurons in primary cell model of Parkinson’s disease induced by 1-methyl-4-phenyl-pyridinium (MPP+). Methods:Embryonic mouse mesencephalic primary neurons were cultured in vitro and divided into four groups:a blank control group, a MPP+ group, a miRNA-NC+MPP+ group, a miRNA-181a+MPP+ group. Dopaminergic neurons dendrites and axons were identified and counted by using tyrosine hydroxlase(TH) immunostaining under microscope in each group. Results:The dopamin(DA) neurons exhibited intact circular or triangular bodies and a variety of dendrites and axons derived from bodies, and had intact morphous and interweaved closely in the control group. The group treated by MPP+ were specifically found that the number of dopaminergic neurons decreased strikingly, axons and dendrites disappeared completely, and bodies remained. The axons appeared abnormal varicosities, apparent string-of-beads change. The axons and dendrites almost fragmented, only few cell bodies disappeared and exhibited axonal root. In the MPP+ group, the number of dopaminergic neurons increased, and cell bodies, axons, dendrites and the death of the dopaminergic neurons became improvement. Conclusion: The results suggested that miRNA-181a may play an important role in protecting dopaminergic neurons in MPP+ induced Parkinson’s disease.

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孔程程,杨文平,华 建,刘 妍,丁海霞.血清miRNA-181a在帕金森病中神经保护作用及机制分析[J].南京医科大学学报(自然科学版英文版),2017,(9):1081-1085.

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  • Received:February 07,2017
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  • Online: September 25,2017
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