Objective: To investigate the function of 3-phosphoinositide-dependent protein kinase-1(PDK1) in the hippocampus dependent learning and memory. Methods: We used the Cre-Loxp to knock out PDK1 in empty spiracles homeobox 1(Emx1) positive cells conditionally, which is expressed in the dorsal telencephalon. PCR was used for genotyping and Morris maze was used to test the learning and memory of mice. Results: We found that the PDK1 knockout mice had weaker capacity of learning and memory associated with hippocampus. Conclusion: PDK1 plays an important role in the hippocampus-dependent learning and memory in mice.