Objective：To investigate the resuscitative potential of mycobacteriophage Guo1 on dormant cells of M.tuberculosis.Methods：Dormant cells of M.tuberculosis were induced by potassium-deficient culture of logarithmic phase of M.tuberculosis at 37 ℃，which were proven through drug sensitivity test，transmission electron microscope(TEM) and auramine O-Nile red double fluorescent staining. M.tuberculosis colony count was used to detect whether the dormant cells of M.tuberculosis had been resuscitated by Guo1. Results：Dormant cells of M.tuberculosis were obtained successfully by potassium-deficient cultivation for 30 days at 37 ℃. The concentration of M.tuberculosis in control，supernatant(SN)，TM4 and Guo1 groups was (2.67±0.58)×102，(3.00±1.00)×102，(2.67±0.58)×102，(2.33±0.58)×102 CFU/mL，respectively，after culture for 1 d. And the concentration of M.tuberculosis in above groups increased to(7.33±1.53)×104，(6.67±2.08)×1010，(3.00±1.00)×1010，(3.33±1.53)×1010 CFU/mL at the 15th day，respectively. Conclusion：Mycobacteriophage Guo1 can resuscitate dormant cells of M.tuberculosis.