Objective: To assess the value of QRS-VHIS interval and QRS-VRVA interval on differentiating the origin of outflow tract premature ventricular contractions. Methods： Sixteen consecutive patients underwent radiofrequency ablation were enrolled from June 2016 to December 2016. Two quadrupolar catheters were positioned at the region of His bundle and right ventricular apex (RVA). With the guidance of the electroanatomic mapping system and fluoroscopy, pace mapping was conducted at different sites in the right ventricular outflow tract(RVOT) and aortic sinus cusp(ASC). Standard 12-lead electrocardiograph(ECG) and intracardiac electrogram of HIS and RVA were recorded simultaneously. Meanwhile, the QRS-VHIS and QRS-VRVA intervals were measured respectively. Results： A total of 139 pacing sites were obtained from 16 patients, and were divided into the RVOT group(n=94) and the ASC group(n=45). The QRS-VHIS interval in the ROVT group was significantly longer than that in the ASC group［(42.98±14.83) ms vs.(19.66 ± 16.14) ms, P<0.001)］. However, there was no significant difference in QRS-VRVA interval between the two groups. Receiver operating characteristic(ROC) analysis showed that the QRS-VHIS interval exhibited a greater area under the curve (AUC) (0.860) than the QRS-VRVA interval(0.626). The QRS-VHIS interval ≤36.4 ms predicted an ASC origin with a sensitivity of 67.1% and specificity of 85.0%. The QRS-VRVA interval ≥57.4 ms had a sensitivity of 54.3% and specificity of 72.0%. Conclusion： The QRS-VHIS interval could be used as a new approach to differentiate the origin of outflow tract premature ventricular contractions.