Objective：To study the expression of CREB1 in glioma and its effect on the proliferation and invasion of human glioma cells. Methods：The expressions of CREB1 in different grades of glioma tissues were analyzed within the Chinese Glioma Genome Atlas expression Dataset（CGGA）. The correlation between CREB1 expression and clinical prognosis in glioma patients was analyzed by using CGGA Dataset. The small interfering RNA of CREB1（si-CREB1）were transfected into U251 and U87 glioma cells, the effect of down-regulation of CREB1 on cell proliferation and invasion were tested by CCK8 assay and Transwell assay. Results：The expression of CREB1 in higher grade glioma tissues was higher than that in lower grade tissues. High-expression of CREB1 associated with unfavourable prognosis in glioma patients. Down-regulation of CREB1 suppressed proliferation and invasion in glioma cells. Conclusion：The expression level of CREB1 relates with tumor pathological grade and clinical prognosis in glioma. si-CREB1 inhibits proliferation and invasion in glioma cells.