Objective：To establish a method of patient derived xenograft（PDX）of human glioblastoma multiforme（GBM）for preclinical and biological research. Methods：GBM samples were freshly collected and planted subcutaneously. After the establishment of subcutaneous xenograft，tumor was taken out，dispersed and injected to the mice brain. HE staining was used to compare the morphological characteristics between primary tumor and PDX tumor，and Ki-67 was detected by immunohistochemistry to compare their proliferation activity. Results：We successfully established the PDX model，and found that the morphological characteristics of PDX by HE staining were similar with primary tumor，PDX remained the proliferation activity of primary tumor. Conclusion：The establishment of PDX model enabled us as preclinical tool for personalized treatment of GBM patients.