Objective：To investigate the expression of lncRNA BX357664 in human renal cell carcinoma （RCC） and its function in epithelial-to-mesenchymal transition （EMT） in Caki-1 and Caki-2 RCC cells. Methods： Downregulated LncRNA BX357664 was confirmed in RCC tumor tissues by previous lncRNA microarray. Real-time PCR was performed to analyze the expression of lncRNA BX357664 in 24 pairs of human RCC samples， Caki-1 and Caki-2 cells， respectively. Transwell migration and invasion assays were performed to detect the ability of migration and invasion in transfected Caki-1 and Caki-2 cells， respectively. Western blotting assay was carried out to identify the relative proteins in EMT process which affected RCC metastasis by lncRNA BX357664. Results： LncRNA BX357664 was downregulated in 24 pairs of RCC samples， Caki-1 and Caki-2 RCC cells， respectively （both P < 0.05）. Overexpression of lncRNA BX357664 suppressed migration （P < 0.01） and invasion （P < 0.01） by inhibiting EMT in Caki-1 and Caki-2 cells. The expressions of EMT related proteins were significantly changed， and the process of EMT was inhibited （P < 0.05）. Conclusion： LncRNA BX357664 was downregulated in RCC and could inhibit the ability of migration and invasion in Caki-1 and Caki-2 cells by blocking EMT.