Objective：To explore the association between single nucleotide polymorphisms（SNPs）in the Wnt signaling pathway genes and the curative effect and prognostic of metastatic colorectal cancer（mCRC）patients who received irinotecan-based chemotherapy. Methods：We selected seven key genes in the Wnt signaling pathway and genotyped the SNPs within seven genes among 110 mCRC patients treated with irinotecan-based chemotherapy. Logistic regression and cox regression analysis were performed to evaluate the association between SNPs and the curative effect and prognostic of mCRC patients. Results：Patients with the LRP6 rs10772542 C alleles had a lower disease control rate（DCR）compared with those with T alleles（OR=2.49，95%CI=1.28~4.83，P=0.007）. LEF1 rs749414 G alleles had a longer progression-free survival（PFS）compared with the T alleles（HR=0.55，95%CI=0.40~0.78，P=0.001）and WNT7B rs10448605 T alleles had a shorter PFS compared with the C alleles（HR=1.65，95%CI=1.13~2.41，P=0.009）. Furthermore，WNT2 rs2239957 C alleles had a longer overall survival（OS）compared with the G alleles（HR=0.54，95%CI=0.35~0.85，P=0.007）. Conclusion：Genetic variants in the Wnt signaling pathway genes contribute to the curative effect and prognostic of mCRC patients who received irinotecan-based chemotherapy.