Objective：To investigate the value of microRNA-122（miR-122）in the evaluation of hepatotoxicity at the early stage. Methods：Based on mouse primary liver cell injury models induced by acetaminophen，the concentrations of miR-122 and traditional cytotoxic marker lactic acid dehydrogenase（LDH）in the cell culture supernatant were measured parallelly，and followed by the correlation analysis. Besides，multiple cellular models induced by different potential hepatotoxic substances were established，and the concentrations of miR-122 and LDH in supernatant were analyzed and compared. The advantage of miR-122 for hepatotoxicity evaluation was further verified. Results：There were well correlations between miR-122 and LDH for hepatotoxicity evaluation（r > 0.9）；Moreover，miR-122 demonstrated higher sensitivity compared with LDH. Conclusion：miR-122 has potential application value as a biomarker for early evaluation of drug hepatotoxicity.