Objective：To explore the effect of miR-29c overexpression on P19 cell proliferation，apoptosis and differentiation. Methods：P19 cells were cultured and transfected with miR-29c mimics. Proliferation was examined with CCK-8 kit and cell cycle was examined with flow cytometey. Apoptosis rate was checked with Hoechst staining and flow cytometry. The mRNA and protein expression of Bax/Bcl-2 was tested with qPCR and Western blot. P19 cells were induced to differentiate with DMSO and the mRNA expression level of αMHC，GATA4，Mef2c was checked with methods of qPCR. We used bioinformatic analysis，lucierase assays and Western blot to find target gene of miR-29c. Results：Compared with the control group，cells in the miR-29c overexpression group showed a lower proliferation rate and lower S cycle percentage. Apoptosis rate of the miR-29c overexpression group was higher than that of the control group. Expression of Bax of the miR-29c overexpression group was significantly higher than that of the control group，while there was no difference seen in Bcl-2 expression. About The mRNA expression level of αMHC，GATA4，Mef2c，the miR-29c overexpression group was significantly higher than those of the control group at day 6 and day 8. Akt3 was proved to be a target gene of miR-29c. Conclusion：Overexpression of miR-29c can inhibits proliferation and promotes apoptosis and differentiation in P19 embryonal carcinoma cells with Akt3.