Objective：To observe the effects of lung protective ventilation strategy combined with dexmedetomidine on oxidative stress response，and postoperative pulmonary complications in patients with pulmonary carcinoma undergoing thoracoscopic radical resection. Methods：Eighty ASAⅠor Ⅱ patients scheduled for thoracoscopic lobectomy were randomly divided into 4 groups（n=20 each）：conventional ventilation group（group C），lung protective ventilation strategy group（group P），dexmedetomidine group（group D）and lung protective ventilation strategy combined with dexmedetomidine group（group P-D）. Arterial blood samples in all groups were collected at baseline（T0），at the time just before OLV（T1），at 30 min after OLV（T2），at 60 min after OLV（T3），at 120 min after OLV（T4）and 15 min after the restoration of TLV（T5）for blood gases analyse and oxygenation index was calculated. The concentration of serum MDA，SOD were measured at the time points of T0，T1，T3，T4，2 hours after operation（T6）and 24 hours after operation（T7）. The incidiences of postoperative pulmonary complications in all groups were recorded. Results：Compared with group C，OI at T5 in group D，at T1~T5 in group P-D was significantly increased（P < 0.05 or P < 0.01）. Compared with group P，OI at T1~T5 in group P-D was significantly increased（P < 0.05 or P < 0.01）. Compared with group C，the concentration of MDA at T4，T6，T7 in group P，at T3，T4，T6，T7 in group D and group P-D was significantly decreased（P < 0.05 or P < 0.01），while the level of SOD at T6，T7 in group P，at T4，T6，T7 in group D and at T3，T4，T6，T7 in group P-D respectively was significantly higher than that of in group C（P < 0.05 or P < 0.01）. Compared with group P，the concentration of MDA at T3，T4，T6，T7 in group P-D was significantly decreased，while the level of SOD at the same time points in group P-D was significantly increased（P < 0.05 or P < 0.01）. Conclusion：Lung protective ventilation strategy combined with dexmedetomidine can further increase oxygenation，attenuate oxidative stress response.