Objective：To establish the dibutyl phthalate（DBP）poisoning mouse model and discuss the protective response of sulforaphane（SFN）. Methods：A total of 30 male mice with age of 6 weeks old were randomly divided into the solvent control group（soybean oil group），the SFN group［0.75 mg/（kg·d）］，the DBP group［500 mg/（kg·d）］，the DBP with SFN low dose group［0.5 mg/（kg·d）］，the DBP with SFN middle dose group［0.75 mg/（kg·d）］and the DBP with SFN high dose group［1 mg/（kg·d）］with 5 mice in each. After continuous feeding drugs for 2 weeks，all mice were sacrificed and anatomized，the testis weight，anogenital distance（AGD），AGD/weight and the organ coefficients of testis were measured. In addition，the testicular histopathological changes of mice were observed and one side of epididymis was used to analysis the sperm counts，viability and deformity rate. Results：The DBP group presented with obvious lower AGD/weight，sperm counts，viability and higher deformity rate，significant testicular histopathological changes compared to the soybean oil group（P < 0.05）. However，all the test results in the DBP with SFN low dose group were superior to that of the DBP group（P < 0.05）and the testicular histopathological changes were improved significantly. Conclusion：SFN could alleviate the reproductive toxicity induced by DBP significantly and this provides an experimental animal model for further studying the toxicity of DBP and the protective mechanism of SFN.