Objective：To explore the effects of KLF7b on embryo development of zebrafish. Methods：Wild-type KLF7b mRNA and KLF7b acetylated site mutant mRNA were transcripted in vitro. Specific mRNA and morpholino oligonueleitides（MOs）were used to overexpress or site mutate or knockdown KLF7b by microinjection at 1~2 cells stage. The phenotypes of embryos were observed at different stages and the expressions of marker genes were tested by quantitative RT-PCR. The bone development of zebrafish was detected by calcein staining. Results：KLF7b overexpression had no effects on embryo development of zebrafish. KLF7b knockdown or acetylated site mutation caused embryonic abnormalities including pericardial effusion，the developmental malformations of somite and head. The marker genes of muscle were down-regulated. Conclusion：KLF7b may affect the development of zebrafish via its own acetylation.