Objective：To investigate the expression of kinesin family member 4A in colorectal cancer and its clinical significance. Methods：Resection samples and related corresponding tissues of colorectal mucosa above 5 cm of 110 colorectal cancer patients were collected. The expression of KIF4A in colorectal tissues and matched adjacent noncancerous tissues was detected by immunohistochemical. The relationship of KIF4A expression with clinicopathological characteristics and follow-up data were statistically analyzed. Results：The expression of KIF4A in colorectal tissues was significantly higher than that in normal tissues（P < 0.001）. The expression of KIF4A was associated with degree of tumor differentiation（P=0.023），lymph node metastasis（P=0.020），distant metastasis（P=0.032），TNM stage（P < 0.001）and serum CEA（P=0.014），but not with gender，age，tumor size（all P > 0.05）. Among the 110 patients with colorectal cancer who were followed，Kaplan-Meier survival curves showed that five year’s survival rate of the high KIF4A expression group was lower than that of the low KIF4A expression group. Univariate survival analysis showed that the expression of KIF4A，degree of tumor differentiation，lymph node metastasis，distant metastasis，TNM stage and serum CEA were associated with the survival time after surgical resection. Multivariate COX proportional hazard regression model analysis further revealed that the expression of KIF4A，TNM stage，lymph node metastasis and distant metastasis were the independent risk factors for prognosis. Conclusion：KIF4A is highly expressed in colorectal cancer tissues，up-regulated expression of KIF4A is associated with metastasis and invasion of human colorectal cancers，which indicates that KIF4A may be valuable in predicting prognosis and could be a potential target for therapy of colorectal cancer.