Objective：To construct a miRNA-205 overexpression vector and study the effect of miRNA-205 on the establishment of porcine induced pluripotent stem cell lines（iPSCs）. Methods：We compared miRNA transcriptional profiles between the primed porcine embryonic stem cells（primed PESCs），porcine inner cell mass（ICM）and pig fetal fibroblast cells（PEF）. The miRNA-205 precursor sequence was amplified by PCR and integrated into the expression vector pCAGDNA3-hFat1 to construct the overexpression vector pCAG-miR205. The expression vector was transfected into porcine fetal fibroblasts containing four mouse derived transcription factors（Oct4，Sox2，Klf4 and c-Myc）by lipofectamine transfection technique. To establish porcine iPSCs，the cells were incubated and cultured in stem cell culture medium. qPCR（real-time quantitative PCR）was used to detect the expression of miRNA-205 in the cells before and after transfection. The inducible effect of miRNA-205 on porcine iPSCs formation was analyzed. Results：The recombinant plasmid pCAG-miR205 was constructed. Compared with untransfected cells，the expression level of miRNA-205 in the transfected cells was significantly increased（P < 0.01），and the colony number was higher after being induced in stem cell culture medium. Conclusion：Over-expression of miRNA-205 at the cellular level can improve the efficiency of porcine induced pluripotent stem cell lineage. The miRNA-205 over-expression vector successfully constructed in this study will help to establish a stable cell line expressing miRNA-205，Which laid the foundation for further study on the exact function and mechanism of miRNA-205 on stem cell reprogramming and pluripotency maintenance.