Objective：To explore the effect of TRIM22 on proliferation，invasion and migration in glioblastoma. Methods：The expression levels of TRIM22 were analyzed in TCGA database and were examined in two glioblastoma cell lines（U87 and U251 cells）using qRT-PCR and Western blot. Two independent TRIM22 siRNAs were transfected into U87 and U251 cells，and the effect of TRIM22 depletion was confirmed. CCK8 assay and colony formation assay were carried out to assess the effect of TRIM22 depletion on glioblastoma cell proliferation. Transwell assay and wound healing assay were also performed to investigate the role of TRIM22 depletion on glioblastoma cell invasion and migration. The expression of p-AKT and epithelial-mesenchymal transition（EMT）-associated proteins including N-cadherin，Vimentin，E-cadherin were examined using Western blot after TRIM22 depletion. Results：Inhibition of TRIM22 expression markedly reduced the proliferation，invasion and migration of glioblastoma cells. Correspondingly，the expression of p-AKT（S473），N-cadherin and Vimentin were decreased，whereas the expression of E-cadherin was increased after TRIM22 depletion. Conclusion：TRIM22 is a crucial factor for regulating the proliferation，invasion and migration of glioblastoma cells through PI3K/AKT signaling.