Objective：To investigate the feasibility of multiphasic computed tomography scanning combined with a homemade software quantitative measurement of glomerular filtration rate（glomerular filtration rate measurement based on computed tomography，CT-GFR）. Methods：Fifty-two patients with unilateral renal tumors from February 2017 to July 2017 were included. All of them did not suffer from acute renal insufficiency. All patients underwent renal CT scans and serum creatinine（serum creatinine，Scr）tests preoperatively，of which 15 cases underwent additional radionuclide examination. Total CT-GFR and relative CT-GFR，with or without renal tumors，were calculated by a homemade GFR quantitative software. The correlation between total CT-GFR and estimated glomerular filtration rate（eGFR）was analyzed by Pearson’s method. The correlation between relative CT-GFR and relative GFR measurement based on radionuclide examination（R-GFR）of split kidneys was also analyzed by Pearson’s method. Total CT-GFR and relative CT-GFR were calculated in early and late renal parenchymal phases respectively with or without renal tumors. Results：Total CT-GFR in early renal parenchymal phase was highly correlated with eGFR（r=0.81，P < 0.01）. Relative CT-GFR in early renal parenchymal phase was highly correlated with relative R-GFR in the split kidneys（r=0.85，P < 0.01）. Conclusion：Split and total GFR can be measured accurately using multiphasic CT combined with homemade software in patients with unilateral renal tumors.