Objective：To detect the efficiency of c-Met CAR infection in T cells by qRT-PCR using specific primers. Methods：Gene recombination technology was introduced to construct c-Met CAR（GFP）retroviral plasmids. C-Met CAR and c-Met CAR（GFP）viruses were prepared by viral packaging. C-Met CAR-T cells and c-Met CAR-T（GFP）cells were prepared by infection of T cells with virus. The expression of c-Met CAR and c-Met CAR（GFP）virus infected 293T cells was tested by Western blot assay to express exogenous CD3ζ protein. Specific primers were designed to detect the infection efficiency of T cells by qRT-PCR and compared with flow cytometry. Results：c-Met CAR（GFP）plasmid was constructed，and the expression of c-Met CAR（GFP）plasmid on 293T cells was observed by fluorescence microscope. The results showed that c-Met CAR and c-Met CAR（GFP）virus infected 293T cells expressed exogenous CD3ζ protein. The infection efficiency of c-Met CAR and c-Met CAR（GFP）virus were（55.9 ± 2.3）% and（52.1 ± 1.7）% by qRT-PCR. The efficiency of c-Met CAR（GFP）infection was（50.7 ± 3.6）% by flow cytometry. There was no statistical difference between the two methods（P > 0.05）. Conclusion：Through the design of specific primers，the qRT-PCR can detect the infection efficiency of c-Met CAR virus on T cells，which is accurate and security，and have value for the clinical application of CAR-T cell therapy.