Objective： By adjusting the phosphorus content in adenine diet，we aimed to establish an effective chronic kidney disease mice model accompanied by hyperphosphatemia for further studies. Methods：Male C57BL/6 mice were divided into four groups：simple adenine dietary：the normal group（1.0% calcium，0.6% phosphorus），the simple adenine group（0.2% adenine，1.0% calcium，0.6% phosphorus），adenine integrating high phosphorus dietary：the normal group（0.8% calcium，0.6% phosphorus），the adenine integrating high phosphorus dietary group（0.2% adenine，0.6% calcium，1.0% phosphorus）（n=7）. The weight，the levels of blood urea nitrogen（BUN），calcium（Ca）and phosphorus（P）in serum were measured at 0 and 4 weeks after the start of adenine diet. The gene expression of fibrosis markers collagenⅠ，fibronectin（FN），plasminogen activator inhibitor-1（PAI-1），in-ammatory markers TNF-α，IL-1β and ICAM-1 in the kidney were detected by RT-PCR. Results：Compared to the control group，their bodyweights were decreased and the serum BUN level［（41.15 ± 4.59）mmol/L］was significantly increased at 4 weeks in the simple adenine group，while the levels of serum Ca［2.62 ± 0.16）mmol/L］and P［（2.22 ± 0.26）mmol/L］were slightly increased（P < 0.05）. In the adenine integrating high phosphorus dietary group，their weights were reduced and the level of serum P［（2.97 ± 0.29）mmol/L］showed significant increase，whereas the level of serum BUN［（14.68 ± 3.57）mmol/L］were slightly increased at 4 weeks（P < 0.05）. There was no significant difference in serum Ca levels. RT-PCR results showed that the expressions of collagenⅠ，FN，PAI-1，TNF-α，IL-1β and ICAM-1 were increased in two model groups. Conclusion：Adenine combined with high phosphorus diet feeding C57BL/6 mice for 4 weeks can establish a hyperphosphatemia model，accompanied by mild renal dysfunction and renal fibrosis，which is an effective method to set up the mice with hyperphosphatemia.