Objective：To study the expression of Netrin-1 in pancreatic tissue in diabetic mice and explore the significance in the development of type 2 diabetes mellitus. Methods：C57BL/6J mice were divided into the normal group and the diabetic model group. RT-PCR and Western blot were used to detect the mRNA and protein expression of Netrin-1 in pancreatic tissue in mice；Ins-1 cells was cultured in medium with normal and abnormal concentration of glucose. MTT was used to detect the cell viability. RT-PCR and Western blot were used to detect the mRNA and protein of Netrin-1 respectively. Results：The mRNA and protein expression of Netrin-1 increased in pancreatic tissue of diabetic mice. While high concentration of glucose up-regulated the mRNA and protein expression of Netrin-1 in ins-1 cells. Conclusion：The level of Netrin-1 increases in pancreatic tissue in diabetic mice which correlates with the abnormal concentration of glucose. Netrin-1 may participate in the pathophysiology of diabetes.