Recombinant human endostatin（rh-Endostatin） is an anti-angiogenic targeting drug with multi-targets. The mechanisms of rh-Endostatin include not only regulating the surface proteins of endothelial cells and intracellular signaling pathways at the microscopic molecular level，but also remodeling the microenvironment of tumors at macro level，and then improving the overall therapeutic effect of tumors. Numerous studies have shown that anti-angiogenic therapy（such as rh-Endostatin） can work together with chemo-radiotherapy，immunotherapy，and other targeted therapies to combat tumors. Our work will give a detailed review of the mechanisms and application of rh-Endostatin and its research progress in different tumors.