Objective：To explore the influence on expression of growth differentiation factor 5（GDF-5）in adipose-derived stem cells（ADSCs）by inhibiting the expression of Noggin. Methods：The recombinant lentivirus vectors of GDF5 gene（Lv-GDF5）and lentivirus vectors carrying Noggin RNAi sequence（Lv-Noggin shRNA）were constructed. ADSCs were divided into four groups：ADSCs in group A were transfected by empty lentivirus（with enhanced green fluorescent protein，Lv-EGFP）；ADSCs in group B were transfected by Lv-Noggin shRNA；ADSCs in group C were transfected by Lv-GDF5；ADSCs in group D were co-transfected by Lv-GDF5 and Lv-Noggin shRNA. Then，the expression of GDF5 and Noggin in ADSCs was determined by real-time PCR and Western blot 14 days after transfection. The capacity of cartilage differentiation was detected through collagen typeⅡ（Col-Ⅱ）by RT-PCR. Finally，the expression quantity was contrastly analyzed. Results：RT-PCR showed that the expression of GDF5 mRNA and protein increased gradually 14 d after transfection from group A to group D. In comparison between each two groups，except the difference between group B and group C was not statistically significant，differences between the other groups were statistically significant（P < 0.05）. The expression of Noggin mRNA and protein increased gradually in group D，B，C and A. In comparison between each two groups，except the difference between group A and group C was not statistically significant，the differences between the other groups were statistically significant（P < 0.05）. The expression of Col-Ⅱ mRNA increased gradually from group A to group D. In comparison between each two groups，the differences were statistically significant（P < 0.05）. The results of Western blot showed that the expression of GDF5 protein increased gradually from group A to group D 14 d after transfection. In comparison between each two groups，the difference was statistically significant（P < 0.05）. The expression of Noggin protein decreased gradually in group A，C，B and D. In comparison between each two groups，the differences between group A and group C，as well as group C and group B were not statistically significant，while the differences between the other groups were statistically significant（P < 0.05）. Conclusion：The expression of GDF5 in ADSCs increased by inhibiting the expression of Noggin. A negative correlation between GDF5 and Noggin expression quantity was found.