Objective：The goal of this study was to compare the signal peptide-CUB（complement C1r/C1s，Uegf，and Bmp1）-EGF（epidermal growth factor）domain-containing protein 1（SCUBE1） levels between acute pulmonary thromboembolism（APTE）patients and healthy subjects and also investigate the value of SCUBE1 combined D-dimer in the diagnosis of APTE. Methods：Thirty-one patients diagnosed with APTE using computed tomographic pulmonary angiography（CTPA）were included in the study as the experimental group. A control group of 19 age-matched，healthy volunteers served as a reference for biochemical parameters. The levels of SCUBE1 from serum in the experimental and control groups were measured，and the Mann-Whitney rank plasma test was used to compare the levels of SCUBE1 in the two groups. The receiver operating curve（ROC）was used to analyze and compare the diagnostic value of SCUBE1，D-dimer for APTE and the combined diagnostic value of both. Results：SCUBE1 levels were significantly higher in the patients with APTE（P＜0.001）. The best cut-off value of SCUBE1 in the diagnosis of APTE was 8.09 ng/mL. The sensitivity of diagnosis of APTE was 87.10% and the specificity was 68.42%. The sensitivity of SCUBE1 combined with D-dimer to diagnose APTE was 81.48%，and the specificity was 88.37%. Conclusion：SCUBE1 combined D-dimer assist in the diagnosis of APTE.