Objective：To analyze pathogenic characteristics of diarrheogenic Escherichia coli（DEC）in the patients with food borne diseases in Jiangsu Province；to understand drug resistance status and provide effective basis for better prevention，control and clinical treatment in the future. Methods：The fecal specimens of diarrhea patients in 48 food borne disease sentinel hospitals in Jiangsu Province were collected in 2017，5 kinds of DEC were detected by traditional separation culture method combined with fluorescence quantitative PCR. The infection characteristics were analyzed by epidemiological description. At the same time，266 DEC strains were subjected to drug resistance test against 14 antibiotics of 8 categories by micro broth dilution method. Results：Among 9 884 samples of fecal or anal swabs，a total of 289 DEC were detected（accounted for 2.92%），the highest detection rate in 5 types was EAEC（accounted for 1.53%），followed by ETEC（accounted for 0.81%）and EPEC（accounted for 0.54%），2 samples were double bacteria combined infection. The positive DEC detection rate was relatively higher in summer，the highest rate was observed in August. The proportions of male and female infection（2.78% and 3.08%，respectively）were approximately equal and no statistical difference was found between different genders（χ2=0.801，P＞0.05）. For age distribution，the highest rate was detected among the 1 year old group（accounted for 4.55%），followed by 1-5-year-old group（accounted for 4.06%），the detection rate of different age groups was statistically significant（χ2=45.780，P < 0.01）. The DEC demonstrated high drug resistance rate（accounted for 91.35%），among which ampicillin had the highest drug resistance rate（64.3%），followed by nalidixic acid（60.9%）and tetracycline（51.9%）；multi-drug resistance rate was 60.9%，1 strains was resistant to all 14 kinds of antibiotics. Conclusion：The infection rate of EAEC in DEC is the highest among food borne DEC in Jiangsu province. Infants younger than 5 years old require emphasized focus. The multi-drug resistance of DEC is relatively serious，which needs to more attention.