Objective：To explore the impact of wogonin on the proliferation and apoptosis of medulloblastoma cell line DAOY.Methods：DAOY cells were divided into experimental groups（added with wogonin at difference concentrations）and control group. The proliferation of DAOY was detected using CCK-8 assay kit．The apoptosis was analyzed by Annexin Ⅴ-FITC/PI double staining and the activity of caspase-3 was determined via colorimetry. Moreover，we also detected the tumor inhibiting function of wogonin in vivo using subcutaneous tumor model. Results：Wognin remarkably inhibited the proliferation of medulloblastoma cell DAOY and induced the apoptosis in a dose and time dependent manner. The apoptosis was characterized by the increase of apoptosis protein caspase-3. Conclusion：Wogonin could inhibit medulloblastoma cell proliferation both in vitro and in vivo via inducing medulloblastoma cells apoptosis.