Objective:To explore the role of fatty acid binding protein 4(FABP4)in colon cancer cell invasion. Methods:The expression of FABP4 in a panel of colon cancer cell lines was detected by ELISA and Western blot. Transwell assays were performed to determine invasion of colon cancer cells. The glucose transporter 1(GLUT1)expression in HT29 cell was knockdown by siRNA and glucose uptake. LDH level and lactate production of colon cancer cells were analysed. Results:The expression of FABP4 in medium supernatant and cells was increased in colcon cancer cells HTCC116,SW620 than that in HT29,Colo-2 and SW116 cells. Overexpression of hrFABP4 promoted invasion in HT29 cells,whereas inhibitor of FABP4 impaired the invasion rate of HTC116 cells. The expression of GLUT1 and aerobic glycolysis of colon cancer cells were upregulated by hrFABP4. Knockdown of GLUT1 or inhibition of aerobic glycolysis in HT29 cell abrogated the effect of hrFABP4 on cell invasion. Conclusion:FABP4 may promotes invasion in colon cancer cells through upregulation of GLUT1 and increased aerobic glycolysis.