Objective：To investigate changes of long non-coding RNA expression in papillary thyroid carcinoma（PTC）and the role of lncRNA in occurrence and development of PTC. Methods：The microarray chip technique was used to detect the lncRNA expression profile in cancer tissues and paracancerous tissues after PTC resection. Five randomly expressed lncRNAs were randomly selected（fold change≥2，P < 0.05）. The real-time PCR method was used to verify expression microarray results. The different expression levels of lncRNA with the clinical features and stage of disease were compared in PTC patients. Results：Gene chip results showed that the expressions of lncRNAs in PTC tissues and paracancerous tissues were significantly different. There were 429 up-regulated lncRNAs and 1 449 down-regulated lncRNAs. In the tumor tissues and paracancerous tissues of 41 patients with PTC，real-time PCR results showed that expression levels of lncRNA TCONS-00020455 and TCONS-00020457（annotated：lncRNA TCONS-00020455 and TCONS-00020457 are abbreviated as lncRNA TCON-455 and TCON-457，and the short names for these two lncRNAs are used in the article.）in PTC tissues were significantly higher than those in adjacent tissues（P < 0.05）. Further analysis found that the expression levels of lncRNA TCON-455 and lncRNA TCON-457 were associated with lymph node metastasis，multiple lesions，and TNM stage in PTC patients（P < 0.05），but not correlated with age and gender（P > 0.05）. Conclusion：There is abnormal expression of lncRNAs in cancerous tissues of PTC patients. High expression of lncRNA TCON-455 and TCON-457 may be associated with metastasis and progression of PTC.