Objective：To investigate the Alzheimer disease（AD）-like changes caused by methamphetamine（METH）exposure，and to elucidate the role of L-type calcium channels in this pathological change. Methods：After primary cultured neurons were exposed to METH（0，30，100，300，and 1 000 μmol/L），Western blotting assay was performed to investigate the expression of AD-like pathological protein amyloid precursor protein（APP）and p-Tau with or without the treatment of nifedipine. Results：After METH treatment，APP and p-Tau increased in a dose-dependent manner. Meanwhile，with a certain concentration of METH cultured with the neurons，the level of APP and p-Tau was increased in a time-dependent manner. After pre-incubation with the calcium channel inhibitor nifedipine（NIF），the METH-induced AD-like changes were significantly improved. Conclusion：METH exposure can cause AD pathological protein changes and L-type calcium channel ihhibitor can partially reverse the adverse changes，therefore，L-type calcium channel may be a potential intervention target for METH with the potential intervention value.