Objective：To study the changes of intestinal organoids function in female 11β-HSD1 knockout mice. Methods：The intestinal tissues of age-sex matched C57BL/6J mice and 11β-HSD1 knockout mice were collected. Fluorescence-activated cell sorting analysis and quantifications of the intestinal epithelial stem cells，progenitor cells and Paneth cells. Real-time quantitative PCR was used to detect the expression of marker genes in stem cells and Paneth cells. Crypt units were isolated for primary organoid culture in vitro，and the percentage of organoid per crypt ratio and the number of crypt-domains per organoid were observed. Immunofluorescence staining was performed to study the localization and quantification of stem cells and Paneth cells among organoids. Results：Compared with the control group，the number of Paneth cells in the small intestine epithelium of the 11β-HSD1 knockout mice was significantly increased，the number of stem cells was not significantly different，the number of progenitor cells was increased，and the expression of the intestinal stem cell marker gene Lgr5 in the intestinal epithelium was elevated. Similar results were observed in the epithelium of the large intestine. The number of large intestine stem cells，progenitor cells and gene Lgr5 expression were increased in the 11β-HSD1 knockout group. In vitro studies showed that the organoid per crypts ratio was increased，and the organoids derived from the 11β-HSD1 knockout group proliferated and differentiated into more complex structures and consist a greater number of crypt-like domains than the wild group. However，there was no significant difference in the results of colonic crypt organ culture. Immunofluorescence staining of stem cells and Paneth cells of small intestinal organs revealed that the intestinal organoids of 11β-HSD1 knockout mice contained more Paneth cells，and the number of stem cells also increased although not significant. Conclusions：The composition of intestinal epithelium changed after 11β-HSD1 mutation. In the small intestinal epithelium，the number of Paneth cells increased significantly. In the large intestinal epithelium，the number of stem cells，progenitor cells and stem cell marker genes increased significantly. In vitro studies have shown that the 11β-HSD1 knockout group contains more Paneth cells，which have more robust proliferation and differentiation ability.