Objective：This study aims to investigate the effect of glucagon-like peptide-1（GLP-1）on phenotypic transformation of aortic valve stromal cells（VIC），suggesting the role of GLP-1 in the development of calcified aortic valve disease（CAVD）and the mechanism of action. Methods：The interstitial cells of porcine aortic valve were isolated and cultured by collagenase digestion. The calcification model of aortic valve interstitial cells was established by high calcium and phosphorus stimulation. The calcification model was treated by different concentrations of GLP-1 . The best concentration of GLP-1 was determined by the calcium ion concentration in the cell lysate. The optimal concentration of GLP-1 was used to stimulate the cells，and then RNA and cell proteins were extracted. The expression of osteopontin（OPN），runt-related transcription factor 2（Runx-2）and related signaling pathway p38 were determined by RT-PCR and Western blot. Results：GLP-1 reduced the concentration of calcium ion in cell lysate and the maximum stimulation concentration of GLP-1 was 200 ng/mL. Compared with the control group，mRNAs and proteins of OPN，Runx-2 in GLP-1 intervention group decreased，and the expression of p65 protein also decreased. Conclusion：GLP-1 can inhibit the calcification and osteogenic phenotype differentiation of VICs by inhibiting the expression of p65.