Objective：To clone the promoter sequences of fat atypical cadherin 1（FAT1），and to preliminarily analyze the transcriptional regulatory mechanism of the promoter. Methods：Promoter region was consructed by bioinformatic methods，and the application of 1 163 bp（-1 029~+134 bp）fragment of 5′upstream sequence of FAT1 gene by PCR was conducted，followed by cloning to pGL3-basic vector to establish the luciferase report gene recombinant plasmid. Another four recombinant plasmids of different lengths were obtained through walking deletion and then cloned to pGL3-basic plasmid as before. The resultant plasmids were transfected into A549 cells and HEK293T cells respectively together with pGL3-basic vector. After this their activities were detected via dual-luciferase reporter assay. Bioinformatic methods was performed to predict the sequences of the potential transcriptional factor binding sites of the core region found in the promoter. Results：The lusiferase reporter gene recombinant plasmids of human FAT1 promoter were successfully conducted. The relative luciferase activities of recombinant promoters，in contrast to pGL3-basic vector，were much higher（P < 0.05）. Note the sequences of the binding sites such as TFAP2C、KLF5 were possibly included in the promoter region（-233~-110 bp）of FAT1 gene，which could bepredicted through bioinformatic means. Conclusion：The construction of the luciferase report recombinant plasmids of FAT1 gene were successfully done，and four of these plasmids had strong luciferase activities in A549 cells and HEK293T cells，comparing to the activities of pGL3-basic plasmid. Through this the core region was probably found. It is concluded the core promoter region of FAT1 gene was possibly located in the（-233~+134 bp）region，in which may preserve potential important transcriptional binding sites.